This is a presentation of Exiqon’s miRCURY LNA™ microRNA Detection Probes for in situ hybridization.miRCURY LNA™ Detection probes have been used successfully in both animal and plant species. A number of peer-reviewed publications have demonstrated the excellent performance of these probes by showing highly specific microRNA expression pattern in different tissues and cells (Figures 4 and 5). See more stunning in situ images here.
Locked nucleic acid (LNA™) nucleosides are a class of nucleic acid analogues in which the ribose ring is “locked” by a methylene bridge connecting the 2’-O atom and the 4’-C atom (Figure 1). LNA™ nucleosides contain the six common nucleobases (T, C, G, A, U and mC) that appear in DNA and RNA and are able to form base pairs according to standard Watson-Crick base pairing rules. However, by “locking” the molecule with the methylene bridge the LNA™ is constrained in the ideal conformation for Wat...